In our recent webinar titled “PK-PD in support of accelerated programmes for antimicrobial development: how much is enough?” with William Hope, we received more questions from the audience than we could answer during the session. We will now follow up on some remaining questions in the forum and encourage our experts but also users to discuss these questions.
Plant extracts are usually tested for antibacterial activity by determining MICs. As plant extracts contain a complex mixture of structurally diverse compounds (sometimes hundreds of different chemical entities), such MICs are not very informative. Before continuing to determine the antibacterial activity, single compounds have to be isolated. Dereplication, access to large amount of bulk plant material, and deciphering synergistic relationships of a multitude of components are major challenges. Drug discovery activities based on plant extracts is extremely challenging and has been so far only rarely successful.
Another problem of complex plant extracts is that they could interfere with the assay resulting in either false positive or false negative results. Therefore is better to start with at least with partially-purified extracts.
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