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Hit confirmation, hit validation

Definition:

Evaluation of a compound discovered to have antibacterial properties (a hit), by confirming its ability to inhibit bacterial growth, assessing its binding to molecular targets of interest, and determining its chemical structure.

Hit validation is essential for eliminating false-positives from high-throughput screens, demonstrating that a compound has the potential to be an effective treatment, with no obvious chemical features that would make it difficult to develop into a drug.