An in vitro closed system that allows to culture bacteria continuously and mimic in vivo infections and drug concentration profiles.

The HFIM is an indispensable tool to characterize in vitro pharmacodynamics of antimicrobial agents by using physiological pharmacokinetic drug profiles. Advantages of the HFIM system also include the testing of a large number of organisms to assess the emergence of resistance, and determining DDIs and combination by controlling the kinetics of multiple drugs.